Figure 4. Release of surface-associated Ebp proteins from E. faecalis cells by a mild (0.5%) SDS treatment.

Western blots of SDS extracts from exponential cells (see text for details) of strain OG1RF and its deletion derivatives and strain DS5. (A) Immunoblot stained with polyclonal anti-EbpA antibodies. (B) Immunoblot stained with polyclonal anti-EbpB antibodies. (C) Immunoblot stained with monoclonal anti-EbpC antibodies. (D) Coomassie staining of the above SDS extraction samples; sample order as in panels A, B and C. EbpA_M, EbpA monomer; EbpA_HMW, high molecular weight EbpA polymers. +SDS, extraction with SDS; -SDS, extraction without SDS. Positions of molecular weight markers are indicated.