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. Author manuscript; available in PMC: 2014 Aug 15.
Published in final edited form as: J Neurosci Methods. 2013 Apr 27;218(1):9–16. doi: 10.1016/j.jneumeth.2013.04.018

Figure 2.

Figure 2

Images of a coronal section through the specimen. A. Blockface image acquired with a Nikon D700 camera (original resolution of 45μm/pixel) immediately before the tissue section was collected; B. The section corresponding to the blockface image, mounted on a large-format (5×7in) glass slide and stained for Nissl substance (Simmons and Swanson, 1993), which allows for the visualization of cell bodies; C. The section adjacent to the blockface image, mounted and stained using a modified Gallyas silver impregnation stain (Gallyas, 1979), which allows for the visualization of myelinated connections between neurons.