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. Author manuscript; available in PMC: 2013 Jul 11.
Published in final edited form as: Science. 2007 Sep 13;318(5848):261–265. doi: 10.1126/science.1146994

Figure 3. GI-FKF1-CDF1 complex associates with CO promoter in vivo.

Figure 3

(A) In vivo interaction between HA-CDF1 and GI-TAP. The 35S‷HA-CDF1 #17 (GI-TAP-) and 35S‷GI-TAP 35S‷HA-CDF1 #17 (GI-TAP+) lines were grown in long days, harvested 4 hours after light onset on day 10, and coimmunoprecipitation assays were performed (7).

(B) HA-CDF1 expression in the 35S‷HA-CDF1 and gi-2 35S‷HA-CDF1 lines. Plants were harvested at day 10 in long days. ACT was used as a loading control.

(C) CO chromatin regions associated with GI-TAP protein. Plants were harvested 13 hours after light onset on day 10. The ratio between the specific enrichment value in the GI‷GI-TAP sample and that in the wild type sample on each amplicon was calculated from 7 independent ChIP analyses (7). ACT2 and UBQ10 genes were used as controls. The dotted line indicates no enrichment.

(D) Schematic drawing of the CO locus and the amplicon locations for ChIP analysis. The 17 amplicon locations are shown. White and light gray boxes represent exons, and 5'- and 3'-untranslated regions (UTR).

(E and F) CO promoter regions associated with FKF1-TAP and HA-CDF1 proteins. Plants were harvested 13 hours [FKF1-TAP (E)] and 4 hours [HA-CDF1 (F)] after light onset on day 10. Data were calculated from 4 independent analyses.