Fig. 3.

Effect of incubation time on cell yield and viability in cold trypsin-EDTA digestion and flow cytometry analysis of single cells

(a) Yields of single cells and ratios of viable single cells to total single cells after incubation of pancreas fragments in trypsin-EDTA fluid for 2, 6, 10, 14, 18, 22, or 24 h at 4 °C (n=5). (b) Flow cytometry analysis of the percentages of the whole cell population (upper left), isotype control (upper right), and ductal (lower left), and BCRP1-positive (lower right) cells in single-cell suspensions prepared by cold trypsin-EDTA digestion for 16 h