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In A. nidulans, peroxisomal localization of A. fumigatus SidI–GFP is blocked by inactivation of PexG (pexG14). Fungal strains were grown in iron-depleted minimal medium containing 200 μM BPS to generate harsh iron starvation for 24 h at 37°C. Upper panel, bright-field image; mid-panel, confocal fluorescence microscopy; lower panel, overlay. Scale bar, 10 μm.
