Fig. 6.

The immature form of CaSR coimmunoprecipitates with 14-3-3 proteins. HEK293 cells transiently expressing wt CaSR, CaSR(5A), or truncations CaSRΔ898(5A) or CaSRΔ868, or the phosphorylation site mutants, S892A/D and S899A/D were subjected to immunoprecipitation with anti-14-3-3 antibody (A) or anti-FLAG antibody (B) as described in methods. Blots from both immunoprecipitations were probed with anti-CaSR LRG antibody or anti-CaSR ADD antibody. C. CaSR immunoreactivity from anti-14-3-3 blot was normalized to that of the same sample from the anti-FLAG blot and plotted as % of wt CaSR. Results are average ± S.E.M. of 3-5 independent transfections.