Fig. 1.

The shrunken population of cells has degraded DNA. S49 Neo cells were treated with dexamethasone (dex; 2.5 × 10^-7 M) for 48 hours. Cells were fixed in 70% cold EtOH overnight, washed in 1X PBS, and stained with propidium iodide for DNA content. Control cells show a single population of cells on a forward-scatter versus side-scatter dot plot that have a normal DNA content. In contrast, cells treated with dex had two distinct populations of cells, with the smaller or shrunken cells having degraded their DNA.