Expression analysis of TASK-1 in the human heart. (A), Quantitative mRNA expression analysis of human TASK-1 in heart tissue pooled from 12 different donors (n = 8 qPCR runs). Relative expression was quantified as 1/2 ‡CT, where ‡CT is CT (GAPDH) - CT (K+ channel). (B), Sample amplification blots of quantitative PCR analysis of human TASK-1 in different cardiac tissues. (C), Quantitative PCR data analyzing the TASK-1 expression in various cardiac regions, normalized to left atrium (n = 5 qPCR runs). For patient information see Table 1 or Material and Methods section.