Fig. 5.

Pendrin E303 missense substitutions show a range of functional impairment. A. ³⁶Cl⁻ influx into oocytes previously injected with water or with 1 ng cRNA encoding wildtype (WT) pendrin, WT pendrin-6His, pendrin E303Q-6His, or the indicated E303 missense mutant pendrin polypeptides without a C-terminal 6His tag. *, p<0.001 WT vs all E303 mutants; p<0.001 WT-6His vs. E303Q-6His. B. ³⁶Cl⁻ influx into oocytes previously injected with water, with 1 ng cRNA encoding WT pendrin or WT pendrin-6His, or with 10 ng cRNA encoding the indicated E303 missense pendrin mutant polypeptides. *, p<0.05 E303Q-6His vs WT-6His; **, p<0.001 vs. WT; C. ³⁶Cl⁻ influx into oocytes previously injected with water or with 10 ng cRNA encoding wildtype 6His (WT) or E303C pendrin in the absence or presence of 10 mM MTSES, 5 mM MTSEA, or 1 mM MTSET. Oocytes were preincubated with MTS reagents 15 min prior to initiation of the influx period.