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Distribution (percent of total metabolites) of individual BaP metabolite types in culture medium and lysates of HT-29 colon cells (106/ml) incubated for 48 & 96 hrs with 10 & 25 μM of benzo(a)pyrene. Post-incubation, cells were lysed by bead-beating treatment; lysates were extracted by LLE and analyzed by HPLC.
