Table 3.

Concentrations (ng/10⁶ cells or ng/ml medium) of BaP parent compound (unmetabolized) in cell cultures. Required concentrations of BaP was added to the cultures and incubated for specified time periods. After incubation, cells were lysed; cells and media were extracted as mentioned in “Materials and Methods” section. The sample extracts were concentrated, reconstituted in methanol, filtered to remove particulates and injected onto a reverse phase HPLC equipped with a fluorescence detector for quantification of BaP.

	Cells				Culture media
Method	10μM		25μM		10μM		25μM
	48 hrs	96hrs	48 hrs	96 hrs	48 hrs	96hrs	48 hrs	96hrs
Solid phase liquid extraction	0.20	ND	0.34	ND	0.30	ND	0.42	0.23
Supported liquid extraction	0.25	ND	0.40	ND	0.18	ND	0.40	0.21
Liquid-liquid extraction	0.40	ND	0.53	ND	0.12	ND	0.30	0.13
Homogeneous liquid-liquid extraction	0.40	ND	0.55	ND	0.15	ND	0.34	0.19