Fig. 9.

Selective inhibition in DUSP6 overexpression HCEC subline (DUSP6+) of EGF and PDBu -induced ERK1/2 and p-NKCC1 phosphorylation. HCEC were serum starved for 24 h at 80% to 90% confluence. Representative Western blot analysis compares p-NKCC1, p-p38, p-JNK1/2/SAPK and p-ERK1/2 formation with the appropriate antibody in cell lysates obtained from wildtype and the DUSP6 overexpressing HCEC subline. Summaries of the individual effects of DUSP6 overexpression are shown below of data (mean ± SEM: n=3; *p<0.05 versus untreated control) obtained from three individual experiments. DUSP6 overexpression selectively blocked EGF and PDBu-induced p-NKCC1 and p-ERK1/2 (n=3; **p<0.001 versus EGF and PDBu-induced p-p38 and p-JNK1/2/SAPK), which is consistent with the notion that NKCC1 phosphorylation is dependent on ERK1/2 activation by either EGF or PDBu.