Table 1.
Oligonucleotide primer sequence
| Purpose | Primer name | Primer sequence |
|---|---|---|
| RT-PCR Dap10 | ZFDAP10-F3 | AGGGCTTCTAGTGTTTCTCCTCTC |
| ZFDAP10-R3 | GCCAAAGATCTTCATGTTTTGCAG | |
| RT-PCR Dap12 | ZFDAP12-F3 | GGATACTGTTTATAATGTTCCCCG |
| ZFDAP12-R3 | TTCCGATACTGCTGAAGATCACTG | |
| RT-PCR Nitr9 | Nitr9-AUG-For | ATGATCAACTTTTGGATTTTTGGACTTTTC |
| Nitr9-TAA-Rev | TTACTGCTGGTTAGAAACCGAGTTAATCAT | |
| RT-PCR TCRα | TCRα For | GGAACACAAGTTCATGTTGAAAC |
| TCRα Rev | GCTCATCCACGCTTTGAAAGTCA | |
| RT-PCR myeloperoxidase | mpx-For | CCAGAACCAGTGAGCCTGAGACACG |
| mpx-Rev | CAGTCTAACCATGGGCAGCGCTGCAC | |
| RT-PCR β-actin | Actin For | GGTATGGAATCTTGCGGTATCCAC |
| Actin Rev | ATGGGCCAGACTCATCGTACTCCT | |
| Myc-Nitr9L | Nitr9-for-blunt | CACCCAAATGCACCACCTGTGTTTGTTAAAC |
| Nitr9-rev-NotIa | gactgcggccgcTTACTGCTGGTTAGAAAC | |
| FLAG-DAP10 | ZFDAP10-For-bluntYSCF | TACTCATGTTTTGGAGTGAGC |
| ZFDAP10-rev-NotIa | acgtgcggccgcTCATGTTTTGCAGTTTGC | |
| FLAG-DAP12 | ZFDAP12-For-blunt | AATCAAGACTGCAGTTCCTGTTACC |
| ZFDAP12-Rev-NotIa | gcatgcggccgcTTATTTCCGATACTGCTGAAG | |
| FLAG-Dap12D42V | ZFDAP12_D→V-topb | GGGATCATCACGTGTGTTATTATTCTCACGCTC |
| ZFDAP12_D→V-botb | GAGCGTGAGAATAATAACACACGTGATGATCCC | |
| FLAG-Dap12Y96A | ZFDAP12_Y→A-topb | GAAGTAGAATCACCTGCCCAGGAGCTTTACGGA |
| ZFDAP12_Y→A-botb | TCCGTAAAGCTCCTGGGCAGGTGATTCTACTTC |
a
Overhang (5′) sequences are in lower case text and include a Not I site.
b
The mutated codons are italicized.