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. Author manuscript; available in PMC: 2013 Jul 12.
Published in final edited form as: J Immunol. 2011 Dec 21;188(3):1360–1370. doi: 10.4049/jimmunol.1101660

Figure 2. IL-17 producing CD4+ T cells progressively increase during brain abscess evolution.

Figure 2

Abscess-associated CD3+ CD4+ T cells were isolated from C57BL/6 mice at the indicated day post-infection by FACS, immediately stimulated ex vivo with PMA + ionomycin for 3 h, and stained for IL-17 or IFN-γ to demonstrate cytokine profiles. (A) Representative dot plots depicting cytokine staining at day 7 post-infection. Note that total CD4+ T cells were sorted and immediately processed for intracellular cytokine staining; therefore, CD4 staining was still present in the isotype controls for IL-17 and IFN-γ. (B) Changes in the percentages of IL-17 and IFN-γ producing CD4+ T cells over the course of infection. Significant differences between the percentages of IL-17- or IFN-γ-positive cells at day 14 are denoted by asterisks (*, p < 0.05). Results represent the mean ± SEM combined from three independent experiments.