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Increased MMP-1, MMP-2, and MMP-3 activity in CAM media. (A) MMP-1, MMP-2, and MMP-3 enzyme activities (using the selective substrates indicated in brackets) are increased in CAM compared with ATM media; left, assays based on Trp fluorescence and right on FRET. (B) CAM-CM media stimulates AGS cell migration and is inhibited by neutralizing antibody to MMP-1 (2.5 μg mL–1) or inhibitors (see text) of MMP-2 (6 μM) and MMP-3 (3 μM). Horizontal bars, p < 0.05.
