Light Regulates BZS1 Accumulation through COP1.
(A) Light represses BZS1 transcription
levels. Etiolated wild-type seedlings were treated with white light for
the indicated time and mRNA levels of BZS1 were
analyzed by qRT–PCR.
(B) Dark induces BZS1 transcription levels.
Light-grown wild-type seedlings were moved into dark for the indicated
time and mRNA levels of BZS1 were analyzed by
qRT–PCR.
(C) Quantitative RT–PCR analysis of
BZS1 expression in the dark-grown wild-type and
cop1 mutants.
(D) Immunoblot analysis of BZS1 protein in 3-day-old
dark-grown OX8 line seedlings treated with blue and red
light for 1 h.
(E) Immunoblot analysis of BZS1 protein levels in dark-grown
wild-type and cop1 mutants.
(F) Dark-grown phenotypes of cop1 mutants
crossed with BZS1–CS1.
(G) Hypocotyl lengths of wild-type, cop1
mutants, CS1;cop1-4, and
CS1;cop1-6. Representatives of the
tallest seedlings of each population were chosen for photographs. Error
bars represent SD (n = 30) and
significant differences from cop1 mutants are marked
(** P < 0.01,
* P < 0.05).
(H)
In vitro pull-down assay showing the interaction
between BZS1 and COP1.