Figure 7.

Light Regulates BZS1 Accumulation through COP1.

(A) Light represses BZS1 transcription levels. Etiolated wild-type seedlings were treated with white light for the indicated time and mRNA levels of BZS1 were analyzed by qRT–PCR.

(B) Dark induces BZS1 transcription levels. Light-grown wild-type seedlings were moved into dark for the indicated time and mRNA levels of BZS1 were analyzed by qRT–PCR.

(C) Quantitative RT–PCR analysis of BZS1 expression in the dark-grown wild-type and cop1 mutants.

(D) Immunoblot analysis of BZS1 protein in 3-day-old dark-grown OX8 line seedlings treated with blue and red light for 1 h.

(E) Immunoblot analysis of BZS1 protein levels in dark-grown wild-type and cop1 mutants.

(F) Dark-grown phenotypes of cop1 mutants crossed with BZS1–CS1.

(G) Hypocotyl lengths of wild-type, cop1 mutants, CS1;cop1-4, and CS1;cop1-6. Representatives of the tallest seedlings of each population were chosen for photographs. Error bars represent SD (n = 30) and significant differences from cop1 mutants are marked (** P < 0.01, * P < 0.05).

(H) In vitro pull-down assay showing the interaction between BZS1 and COP1.