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. Author manuscript; available in PMC: 2013 Jul 12.
Published in final edited form as: Biochem J. 2009 Oct 12;423(3):411–419. doi: 10.1042/BJ20090543

Table 2.

The binding specificity of HsPP5 TPR binding T7 phages monitored at 405 nma.

Phage
Sequence		 #1
RFQQMNQSWDVD 		#2
RSHGEGWLDAVD 		hsp90 C-12
GDDDTSRMEEVD 		LFG#1
RLLSADDDVLFG
Protein
FL PP5 0.10 0.17 0.08 0.02
PP5 TPR 0.88 0.60 0.58 0.48
FL Hop 0.74 0.56 0.66 0.00
Hop TPR 1 (70)b 0.75 0.30 0.00 0.00
Hop TPR 2A 0.26 0.82 0.65 0.20
Hop TPR 2B 0.22 0.58 0.58 0.22
FL FKBP51 0.00 0.00 0.00 0.00
FKBP51 TPR 0.05 0.09 0.09 0.01
FL FKBP52 0.00 0.00 0.00 0.00
FKBP52 TPR 0.02 0.05 0.02 0.03
Cyp40 TPR 0.04 0.12 0.03 0.01
a

The phage concentration was 1.7 × 1010 pfu/ml. The incubation time for the ELISA experiments was 30 min.

b

Hop TPR1 is hsp70 specific, the other proteins and constructs are capable of binding to hsp90.