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. Author manuscript; available in PMC: 2013 Jul 12.
Published in final edited form as: Biochem J. 2009 Oct 12;423(3):411–419. doi: 10.1042/BJ20090543

Table 3.

Identification of RnPP5 TPR residues required for optimal T7 phage binding a.

Phage
Sequence		 #1
RFQQMNQSWDVD 		hsp90 C-12
GDDDTSRMEEVD 		LFG#1
RLLSADDDVLFG 		LFG#2
KHTVADDELLFG
Wild Type 1.14 1.26 0.95 0.89
E29A 0.91 1.06 0.77 0.75
K32A 0.96 0.09 0.74 0.76
K40A 1.01 0.95 0.85 0.90
E56A 0.96 1.08 0.78 0.77
I63A 0.84 0.83 0.79 0.83
R74A 0.75 0.24 0.69 0.75
E76A 0.98 1.12 0.89 0.93
Y80A 1.08 1.09 0.76 0.73
K97A 0.15 0.02 0.60 0.62
R101A 0.91 0.00 0.53 0.54
R113A 0.84 0.72 0.66 0.74
R117A 1.00 1.06 0.80 0.78
a

The phage concentration was 1.5 × 1010 pfu/ml. The incubation time for the ELISA experiments was 30 min.