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. Author manuscript; available in PMC: 2013 Jul 12.
Published in final edited form as: Anticancer Res. 2011 Oct;31(10):3129–3135.

Figure 1. Differential effects of HRG on AR expression in C81 vector and EBP1 transfectants.

Figure 1

A: C81 vector (V) and EBP1-transfected (E) cells were serum-starved overnight and treated with HRGß1 (20 ng/ml) for 24 hours. Cell lysates were analyzed by Western blotting for expression of AR and actin as indicated. A representative Western blot of three independent experiments is shown. B: Densitometric analysis of the blot in A. C: Total RNA was extracted from vector control and EBP1 transfected C81 cells treated with HRG (20 ng/ml, 24 hours) or EGF (50 ng/ml, 24 hours) to detect steady-state levels of AR and actin mRNA by RT-PCR. A representative gel of three independent experiments is shown.