Skip to main content
. Author manuscript; available in PMC: 2013 Jul 12.
Published in final edited form as: Anticancer Res. 2011 Oct;31(10):3129–3135.

Figure 3. HRG attenuates AR mRNA translation only in EBP1 transfected cells.

Figure 3

C81 vector and EBP- transfected cells were serum-starved overnight and then treated with HRG (20 ng/ml) for 24 h. Cell lysates were processed as described in the Materials and Methods. A: Absorbance profiles at 254-nm. B: Western blot analysis of polysome fractions. C: The levels of AR and actin mRNAs in each gradient fraction were measured by RT-qPCR and plotted as a percentage of the total AR (left panel) or actin mRNA levels (right panel) in that sample. Data represent one of three independent experiments showing similar results.