Table 1. Spot complementation tests with amber phage in genes 4 and 5.

Plasmid containing strains were tested for their ability to support the growth of wild type phage or phage with amber mutations in the major capsid protein gene (5) or the protease gene (4), as described in Materials and Methods.

Plasmid	gene 5 am relative EOP	gene 4 am relative EOP	HK97 wild type relative EOP
wild type gp4-gp5	1*	1*	1*
gene 4 frameshift	1	0.001	1
gene 4 LIM45	1	0.001	1
gene 4 LIM50	1	0.001	1
gene 4 LIM78	1	0.001	1
gene 4 LIM88	1	0.001	1
gene 4 LIM130	1	0.001	1
gene 4 LIM140	0.3	0.001	1
gene 4 LIM179	1	0.5	1
gene 4 H65A	0.1	0.001	1
wild type gp4-gp5 induced	1*	1*	1*
wild type gp4-gp5 uninduced	0.1	0.01	1
gene 4 frameshift induced	1	0.001	1
gene 4 frameshift uninduced	0.01	0.01	0.1
6His-gp4–gp5 induced	0.1	0.1	0.1
6His-gp4–gp5 uninduced	1	1	1

The values are all relative to the complementation achieved by wild-type versions of genes - these are marked with a”*”.