Fig. 3.
Stimulation of H+-ATPase activity requires an intact microtubular network. Isolated CNT-CCDs were preincubated with colchicine (20 μM) for 20 min prior to stimulation with 10 nM angiotensin II. (A) Original pHi tracing of an intercalated cell incubated with colchicine and angiotensin II. (B) Summary of pHi recovery rates of intercalated cells under control conditions, angiotensin II, and colchicine with angiotensin II. pHi recovery rates from control and angiotensin II are taken from Fig. 1 and shown for comparison. Mean ± SEM, ***indicates p < 0.001 compared to angiotensin II alone.