Table 2.
Summary of functional data from pH measurements.
Initial pHi | Na+- independent pH, recovery (ΔpH/min) | Na'-dependent pH, recovery (ΔpH/min) | Final pHi | Number Cells (tubules) | |
---|---|---|---|---|---|
Control | 7.29 ± 0.01 | 0.037 ± 0.003 | 0.184 ±0.012 | 7.24 ± 0.02 | 36(7) |
Concanamycin | 7.31 ±0.01 | 0.006 ± 0.001 | 0.262 ±0.018 | 7.23 ± 0.02 | 53(5) |
Angiotensin II | 7.27 ± 0.03 | 0.088 ± 0.007 | 0.351 ±0.031 | 7.26 ± 0.04 | 20(6) |
Angiotensin II Concanamycin | 7.27 ± 0.01 | 0.009 ± 0.001 | 0.262 ± 0.039 | 7.23 ± 0.04 | 35(5) |
Saralasin Angiotensin II | 7.29 ± 0.02 | 0.031 ±0.007 | 0.189 ±0.023 | 7.19 ±0.03 | 24(4) |
Colchicine Angiotensin II | 7.33 ± 0.02 | 0.032 ± 0.002 | 0.119 ±0.055 | 7.18 ±0.02 | 22(4) |
Data are summarized as mean ± SEM. Shown are initial pHi in the presence of sodium, pHi recovery rates after removal of NH4Cl in the absence of sodium and after readdition of sodium to the bath, final pHi before calibration, and the number of intercalated cells and isolated CNT-CCDs investigated.