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. 2013 Jun 13;4:1954. doi: 10.1038/ncomms2954

Table 2. Cyclic electron transfer rates in the wild type, stt7 and ptox2 strains in oxic and anoxic conditions.

  Cyclic electron transfer rates (e s−1 per PSI)±s.d.
  Oxic conditions Anoxic conditions
WT
 56 μE m−2 s−1 14±3 (PS II) 35±4 (PS I)
 135 μE m−2 s−1 17±2 (PS II) 40±5 (PS I)
     
stt7
 56 μE m−2 s−1 17±2 (PS II) 32±4 (PS II)
 135 μE m−2 s−1 17±3 (PS II) 33±5 (PS II)
     
ptox2
 56 μE m−2 s−1 17±5 (PS I) 30±4 (PS I)
 135 μE m−2 s−1 22±4 (PS I) 33±3 (PS I)

The flux associated with cyclic electron transfer was measured as in the study by Alric et al.32 using DCMU to inhibit LEF. To insure that the measured fluxes were not kinetically limited by photochemical turnovers, we used two different light intensities: 56 and 135 μE m−2 s−1 that correspond to Photosystem I turnovers of ~60 and 150 e−1s−1 per PSI, respectively (see text and Fig. 3, for a detailed description of the methods allowing these estimates). In both cases, the photochemical rate was thus twice- to four-fold larger than the measured CEF rate, so that cyclic electron transfer was the limiting step in the process. In each case, the association of LHCII mobile antenna to PS II or PSI is highlighted by (PS II) or (PSI), respectively. The data are the average of nine independent experiments.