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. 2013 Jun 17;4:1967. doi: 10.1038/ncomms2967

Figure 2. Microtubule nucleation on existing phragmoplast microtubules.

Figure 2

(a) Recovery of microtubules after propyzamide treatment. A cell expressing GFP-α-tubulin and EB1-GFP was treated with 5 μM propyzamide for 8 min, and then culture medium was replaced with a drug-free medium. The left panel shows a low magnification picture of the observed cell. The boxed region of the panel is shown in other panels. In the left panel scale bar, 5 μm. (b) Immuno-cytochemical localization of α-tubulin and γ-tubulin in the phragmoplast of a control cell. Scale bar, 5 μm. (c) Localization as for (b) in a cell treated with propyzamide for 3 min. Scale bar, 5 μm. (d) A cell expressing γ-tubulin-GFP imaged just before and 2 min after 5 μM propyzamide treatment. (e) Effect of anti-γ-tubulin antibody injection on GFP-α-tubulin distribution in the phragmoplast. Scale bar, 5 μm. (f) Effect of anti-γ-tubulin antibody injection on GFP-EB1 distribution in the phragmoplast. Regions around midzones are indicated by yellow boxes (insets). mid, midzone. e, leading edges of the phragmoplasts. Scale bar, 5 μm. (g) Micro-domain structure of microtubules in the phragmoplast based on our results and Ho et al.21 Plus and minus ends of microtubules are shown as+and −.