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. 2013 Jun 18;4:1996. doi: 10.1038/ncomms2996

Figure 2. Secretion components localize similarly in vegetative and primary hyphae.

Figure 2

Fluorescent secretory components (Inline graphic) were imaged in M. oryzae filamentous vegetative hyphae in vitro (a,b,d,f) and in tubular primary hyphae growing in rice cells (c,e,g). BICs (white arrows) were observed adjacent to the primary hyphal tips even without fluorescent labelling. (a) Spitzenkörper marker Mlc1:GFP (green) co-localizes (yellow) with the endocytic tracker dye FM4-64 (red) at tips of vegetative hyphae. This confocal image was acquired 10 min after FM4-64 addition. Images clockwise from upper left are bright-field; GFP; merged GFP and FM4-64; and FM4-64 alone. Similarly, Mlc1:GFP identifies Spitzenkörper in primary hyphae inside rice cells (Fig. 3a). (b–g) Images from left to right are bright-field; and GFP alone. (b) Polarisome component Spa2:GFP localizes to the tip of a vegetative hypha. (c) Polarisome component Spa2:GFP concentrates at the primary hyphal tip behind the BIC at 22 h.p.i. (d) v-SNARE GFP:Snc1 localizes at the vegetative hyphal tip. (e) v-SNARE GFP:Snc1 localizes in a bright fluorescent punctum near the primary hyphal tip at 24 h.p.i. (f) Exocyst component Exo70:GFP localizes at the vegetative hyphal tip. (g) Exocyst component Exo70:GFP localizes at the primary hyphal tip at 24 h.p.i. Scale bars, 5 μm.