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Determination of ΔtrpE tryptophan auxotrophy. Washed, starved, exponential-phase PA14, ΔphnAB and ΔtrpE strains were grown in MOPS minimal medium with no tryptophan. Wild-type growth was observed for ΔphnAB; however, ΔtrpE did not grow until the addition of tryptophan to a final concentration of 200 µM after three hours of non-growth. ΔtrpE did not grow in the absence of tryptophan.
