Fig. 3.

PQS production in the presence of aromatic amino acids and anthranilate. (a) PQS was extracted and quantified from whole cultures of PA14, ΔphnAB, ΔkynA and ΔtrpE ΔkynA grown for 24 h in MOPS minimal medium supplemented with 5 mM of the indicated aromatic amino acids. ND indicates no detectable levels of PQS and error bars represent sem. (b) PQS was extracted from whole cultures of ΔphnAB grown for 24 h in MOPS minimal medium supplemented with 5 mM anthranilate or serine. Extracts were separated by TLC and imaged under long-wave UV light. Extractions from three independently grown cultures are shown for anthranilate and serine addition. 250 ng synthetic PQS (PQS) or anthranilate (AA) were included as TLC migration standards.