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Overexpression of anthranilate synthase genes results in pathway crosstalk. (a) Overexpression of phnAB in ΔtrpE restored growth in MOPS minimal medium without tryptophan, while empty vector did not. (b) Overexpression of fused trpEG restored the ability of ΔphnAB to generate PQS in a minimal medium, while empty vector did not. ND indicates no detectable levels of PQS and error bars represent sem.
