Table 1. Strains and plasmids used in this study.
All mutant strains and plasmids were generated in this study unless otherwise referenced.
| Strain or plasmid | Description |
| Strains | |
| E. coli | |
| DH5α | Wild-type strain for molecular cloning (Sambrook & Russell, 2001) |
| SM10 | Conjugation strain for deletion mutant generation (de Lorenzo & Timmis, 1994) |
| P. aeruginosa | |
| PA14 | Wild-type Pseudomonas aeruginosa strain (Liberati et al., 2006) |
| ΔtrpE | trpE clean deletion in PA14 background |
| ΔkynA | kynA clean deletion in PA14 background |
| ΔphnAB | phnAB clean deletion in PA14 background |
| ΔtrpE ΔkynA | trpE and kynA double clean deletions in PA14 background |
| ΔtrpE ΔphnAB | trpE and phnAB double clean deletions in PA14 background |
| ΔtrpE ΔpqsA | trpE and pqsA double clean deletions in PA14 background |
| Plasmids | |
| pEX18Tc | Gene replacement vector, TcR (Hoang et al., 1998) |
| pJN105 | araC-pBAD expression vector, GmR (Newman & Fuqua, 1999) |
| pGPKO-trpE | pEX18-derived trpE deletion vector, TcR |
| pGPKO-kynA | pEX18-derived kynA deletion vector, TcR |
| pGPKO-phnAB | pEX18-derived phnAB deletion vector, TcR |
| pGPKO-pqsA | pEX18-derived pqsA deletion vector, TcR |
| pGP-trpEG | pJN105-derived trpEG overexpression vector, GmR |
| pKP-phnAB | pJN105-derived phnAB overexpression vector, GmR |