Table 1. Bacterial strains and plasmids used in this study.
Strain or plasmid | Relevant genotype | Source or reference |
Strains | ||
P. gingivalis | ||
W83 (wild-type) | ATCC BAA-308 | |
DEL0121 : : Erm insertion–deletion mutant (Emr) strain W83 | Alberti-Segui et al. (2010) | |
PgTcKI_1 (Tcr); W83ΔPG0121 : : Erm complemented with PG0121 knock-in strain; Tcr | This study | |
E. coli* | ||
BL21-DE3+pET22b with PG0121 HU | Alberti-Segui et al. (2010) | |
SG 632 | MG1655 wild-type | Dr Steven D. Finkel, USC† |
SG 790 | MG1655 hupB : : Km hupA : : Cm | This study |
SG 919 | MG1655 hupB : : Km hupA : : Cm+pTrcHis2C | This study |
SG 883 | MG1655 WT+pTrcHis2A | This study |
SG 886 | MG1655 hupB : : Km hupA : : Cm+pTrcHis2A | This study |
SG 918 | MG1655 hupAB double mutant+pTrcHis2C expressing tagless PG0121 HU coding region | This study |
SG 903 | MG1655 hupAB double mutant+pTrcHis2A expressing tagless E. coli HUβ coding region | This study |
Plasmids | ||
pT-COW | Cbr, Tcr | Gardner et al. (1996) |
pTrcHis2A and C | Ampr | Invitrogen Life Technologies |
pET22b | Ampr | Novagen |
The SG number represents the internal reference number for the Goodman Lab.
USC, University of Southern California.