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. 2013 Feb;159(Pt 2):219–229. doi: 10.1099/mic.0.061002-0

Table 1. Bacterial strains and plasmids used in this study.

Strain or plasmid Relevant genotype Source or reference
Strains
P. gingivalis
W83 (wild-type) ATCC BAA-308
DEL0121 : : Erm insertion–deletion mutant (Emr) strain W83 Alberti-Segui et al. (2010)
PgTcKI_1 (Tcr); W83ΔPG0121 : : Erm complemented with PG0121 knock-in strain; Tcr This study
E. coli*
BL21-DE3+pET22b with PG0121 HU Alberti-Segui et al. (2010)
SG 632 MG1655 wild-type Dr Steven D. Finkel, USC†
SG 790 MG1655 hupB : : Km hupA : : Cm This study
SG 919 MG1655 hupB : : Km hupA : : Cm+pTrcHis2C This study
SG 883 MG1655 WT+pTrcHis2A This study
SG 886 MG1655 hupB : : Km hupA : : Cm+pTrcHis2A This study
SG 918 MG1655 hupAB double mutant+pTrcHis2C expressing tagless PG0121 HU coding region This study
SG 903 MG1655 hupAB double mutant+pTrcHis2A expressing tagless E. coli HUβ coding region This study
Plasmids
pT-COW Cbr, Tcr Gardner et al. (1996)
pTrcHis2A and C Ampr Invitrogen Life Technologies
pET22b Ampr Novagen
*

The SG number represents the internal reference number for the Goodman Lab.

USC, University of Southern California.