Figure 8.
CpG methylation analysis at the upstream USF binding site. (A) Effects of the demethylating reagent DAC on the exon 1a transcription. Cells were treated with 5 μM DAC for 4 days, and the RNA was isolated and amplified using exon 1a-specific primers. (B) Correlation between the upstream E box methylation and the exon 1a transcript expression in various cells.