Fig. 5.

Requirement of residues within the C-terminal region of domain II for genome replication in the JFH-1 and Con1 isolates. The effect of alanine mutation on RNA replication was screened by a colony-forming assay (Con1) (Tellinghuisen et al., 2008b) or by luciferase reporter/qRT-PCR (JFH-1; this study). Red, non-replicating; orange, partial and statistically significant impairment of replication; green, no effect on replication. The lower part of the figure shows the consensus across this region based on a subset of isolates representing each genotype (Simmonds et al., 2005). A full alignment of these sequences is shown in Fig. S1. Filled bars indicate the percentage conservation at each residue, whilst asterisks denote residues that are 100 % conserved.