Fig. 2.

PrP^CWD in the brain and upper respiratory tract epithelia. (a–c) IHC detection (RedMapstaining) demonstrated PrP^CWD as punctate dots (long arrows) and coarse aggregates (short arrows) in the midbrain (a, b) and brainstem ganglion (c) of a ferret inoculated by the n.ph. route. There was symmetrical distribution of PrP^CWD in the thalamic region. PrP^CWD was associated with neurone support cells (short arrows) and a spongiform lesion (*) of brainstem ganglion. (d) PrP^CWD in the olfactory cell layer and in unmyelinated nerves (long arrows) beneath the olfactory epithelium of ferret no. 538 inoculated by the i.c. route. PrP^CWD was associated with the basal cell layer (open arrows) adjacent to the basement membrane (dotted line). (e, f) PrP^CWD associated with the olfactory cell layer of ferret nos 521 (e) and 540 (f), inoculated by the i.g. and n.ph. routes, respectively. (g) Olfactory epithelium of a CWD-negative ferret. Bars, 500 µm (a); 100 µm (c, f, g); 50 µm (d, e).