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Effects of tetherin expression on virus particle production. (a) MDCK cells transduced with a lentiviral vector expressing Flag-Tetherin or with an empty vector (Flag-Teth +/−) were infected (m.o.i. = 10) with or without SeV (SeV+/−) for 24 h. Cell lysates and viral particles were analysed as in Fig. 1 by Western blots. (b) Titres of released infectious virus by flow cytometry. Mean of two separate experiments with deviation from the mean.
