Fig. 1.

Cloning strategy utilized to construct FMDV infectious copy plasmids encoding the Renilla luciferase protein (RL) or different portions of GFP. (a) Schematic representation of the FMDV genome and encoded protein products. (b) Schematic representation of the FMDV genome site utilized for the insertion of the RL gene or different portions of the GFP gene. The retained 3C cleavage site at the C terminus of VP1 and the amino acids (underlined) encoded by the introduced AvrII restriction endonuclease site used for cloning the insertions are shown. The RL and different GFP insertions are depicted below, along with their respective nomenclature (T2- to T7-FMDV, GFP-FMDV, RL-FMDV), nt length (in parentheses) and encoded peptide.