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. 2013 Jul;94(Pt 7):1517–1527. doi: 10.1099/vir.0.052308-0

Fig. 5.

Fig. 5.

Detection of iLOV-FMDV-infected cells by flow cytometry. Goat epithelium cells were either mock infected or infected with iLOV-FMDV or parental FMDV at an m.o.i. of 2. After 4 h, cells were analysed by flow cytometry. (a–h) Density plots for iLOV-FMDV-infected or parental-FMDV-infected cells. (a, b) Infected and unlabelled cells analysed for iLOV expression. (c, d) Infected cells analysed for iLOV and FMDV non-structural protein 3A expression as detected with mAb 2C2. (e, f) Infected cells analysed for iLOV and FMDV capsid expression as detected with mAb IB11. (g, h) Infected cells analysed for iLOV expression and labelled with isotype control mAbs TRT1 and TRT3. (i–l) Histograms displayed for unlabelled cells infected with parental FMDV (i), mock infected (j), infected with iLOV-FMDV (k) and overlay of parental-FMDV-infected (red) and ILOV-FMDV-infected (black) cells (l).