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Replication of rMP12-TOSNSs in cell culture. (a) MEF cells, (b) VeroE6 cells or (c) MRC-5 cells were mock-infected or infected with MP-12 or rMP12-TOSNSs at an m.o.i. of 0.01. Culture supernatants were collected at indicated time points, and virus titre was determined by plaque assay with VeroE6 cells. Means±sd of at least three independent experiments are shown in each graph. Asterisks represent statistical significance (Unpaired Student's t-test, *P<0.05 and **P<0.01 vs MP-12).
