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. 2013 Feb 26;54(4):649–662. doi: 10.1093/jrr/rrt010

Table 1.

Comparisons of the protein ratio obtained from western blots and SILAC-MS analysis

Protein name Normalized ratio of western blot
Mean ASAP ratio of SILAC
15 h/Ctrl 48 h/Ctrl 15 h/Ctrl 48 h/Ctrl
XRCC5 0.80 ± 0.21 0.68 ± 0.18 0.86 ± 0.24 0.49 ± 0.25
XRCC6 0.71 ± 0.19 0.66 ± 0.04 0.64 ± 0.54 0.77 ± 0.11

Gray intensity analysis for western blot was measured with Photoshop CS. The value was then normalized using the levels of β-actin. Average normalized value was presented from three independent experiments. MS = mass spectrum.