Fig. 3.

Binding activity of mAbs AGP127-8 and MGP72-17 and anti-MARV GP rabbit serum to WT and mutant MARV GPs in ELISA. Serially fourfold diluted mAbs AGP127-8 (a) and MGP72-17 (b) and anti-MARV GP rabbit serum (FS0505) (c) were examined for their binding activities to HEK293T cells expressing WT or variant MARV GPs. Cells transfected with the vector only (pCAGGS) were used as the mock control antigen. Rabbit serum FS0505 was used to examine the expression levels of GPs because it recognizes the epitope (aa residues 67–81 of MARV GP) separately from the region where the mutations were found in the GP of escape variants. Experiments were performed three (a and b) or two (c) times; mean values and sd are shown.