Fig. 5.

Proteolytic cleavability of WT and mutant MARV GPs. HEK293T cells were co-transfected with pCAGGS expressing MARV GP and VP40. Produced VLPs bearing WT or mutant MARV GPs were analysed by 7.5 % SDS-PAGE, followed by Western blotting. The uncleaved GP and the GP2 subunit were detected using mAb MGP14-22 that recognizes the epitope located in the GP2 subunit. The experiment was performed three times; a representative dataset is shown.