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. 2013 Jun;159(Pt 6):1023–1035. doi: 10.1099/mic.0.067116-0

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Fig. 1. — Sequence alignment of phages ϕEf11, ϕFL1C and spontaneous recombinant phage [ϕEf11(Δ61-1, ϕFL1C 40-44)] in the region of recombination (ϕEf11 ORFs 60/61-1 and ϕFL1C ORFs 39/40–44). Red indicates ϕEf11 sequences, green indicates ϕFL1C sequences. Sp, Spontaneous recombinant. Genomic coordinates are indicated to the right of each row of sequence. Sites of sequence identity between ϕEf11 and ϕEf11(Δ61-1, ϕFL1C 40-44) are indicated by asterisks. (a) Overview of the regions of ϕEf11 and ϕFL1C that recombined to yield recombinant ϕEf11(Δ61-1, ϕFL1C 40-44). (b) ϕEf11 sequence from 39 307 (within ORF 60) to 451 (within ORF 1), and ϕFL1C sequence from 14 236 (within ORF 39) to 17 451 (within ORF 44). The segment of the ϕEf11 sequence that has been replaced by the ϕFL1C sequence to form the ϕEf11(Δ61-1, ϕFL1C 40-44) recombinant is indicated by Graphic 1. NdeI restriction site in ϕEf11 sequence is indicated.

Fig. 1. — Sequence alignment of phages ϕEf11, ϕFL1C and spontaneous recombinant phage [ϕEf11(Δ61-1, ϕFL1C 40-44)] in the region of recombination (ϕEf11 ORFs 60/61-1 and ϕFL1C ORFs 39/40–44). Red indicates ϕEf11 sequences, green indicates ϕFL1C sequences. Sp, Spontaneous recombinant. Genomic coordinates are indicated to the right of each row of sequence. Sites of sequence identity between ϕEf11 and ϕEf11(Δ61-1, ϕFL1C 40-44) are indicated by asterisks. (a) Overview of the regions of ϕEf11 and ϕFL1C that recombined to yield recombinant ϕEf11(Δ61-1, ϕFL1C 40-44). (b) ϕEf11 sequence from 39 307 (within ORF 60) to 451 (within ORF 1), and ϕFL1C sequence from 14 236 (within ORF 39) to 17 451 (within ORF 44). The segment of the ϕEf11 sequence that has been replaced by the ϕFL1C sequence to form the ϕEf11(Δ61-1, ϕFL1C 40-44) recombinant is indicated by Graphic 1. NdeI restriction site in ϕEf11 sequence is indicated.