Plaque assay of ϕEf11 wild-type (wt), spontaneous recombinant [(ϕEf11(Δ61-1, ϕFL1C40-44)] and virulent mutant [ϕEf11(vir)PnisA]. (a) wt after incubation for 1 day, (b) wt after incubation for 2 days, (c) spontaneous recombinant after incubation for 1 day, (d) spontaneous recombinant after incubation for 4 days, (e) virulent mutant after incubation for 1 day, (f) virulent mutant after incubation for 4 days.