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. 2013 Jun;159(Pt 6):1023–1035. doi: 10.1099/mic.0.067116-0

Table 2. PCR primers used in this study.

Primer Sequence (5′→3′) Use
EF31UF GATAGTTCTTGTTTCGACAAATCAC Amplify upstream of ϕEf11 ORF 31
EF31UR CTGTCGACGTTCCTGCAGAGCTCTAAATAAATATGGCAAGTA Amplify upstream of ϕEf11 ORF 31
EF37DF CTGGATCCATGTGCTATGATTACTCAAAATTAGCAG Amplify downstream of ϕEf11 ORF 36
EF37DR CTGCATGCCCTTTACCAGTAATTTTCGGCGT Amplify downstream of ϕEf11 ORF 36
RKnpF CTCCATGGTCTCTCCTGCAGATAGAATTCTCATGTTTGACAGCTTATCA Amplify nisR and nisK
RKaxR CTGCATGCTCTCTCGACGTCGCCAGTTAATAGTTTGCCGAA Amplify nisR and nisK
PNISaF CTGACGTCACAAAAGCGACTCATAGAATTATTTCCTCC Amplify Erm-PnisA
PNISR GCTTATCGAAATTAATACGACTCACTATAGG Amplify Erm-PnisA
EF31UUF AAGAGCACCTCAAATTCCAGT Detection of ϕEf11 ΔORFs 31–36 (upstream)
RK5R TGATAAGCTGTCAAACATGAGAATTCT Detection of ϕEf11 ΔORFs 31–36 (upstream)
37DDR TGTGATTTGCATGTAGACATCTCCT Detection of ϕEf11 ΔORFs 31–36 (downstream)
PNIS3F TTGTAAAACAGGAGACTCTGCATG Detection of ϕEf11 ΔORFs 31–36 (downstream)
EF31MF AAGTTGTTTCCGTGTCAACGTGGC Detection of ϕEf11 ORF 31 deletion
EF31MR GTGTCCATCATGGTCGTTTAGCAG Detection of ϕEf11 ORF 31 deletion
EF36MF TTATCAGGGTCTGGTGAATGCG Detection of ϕEf11 ORF 36 deletion
EF36MR GCAACTTATGAGTGAGCGCAA Detection of ϕEf11 ORF 36 deletion
ϕEf11F GAGAGTGGAAGTGGATTCAATG Detection of ϕEf11 ORF 43
ϕEf11R GCACTTTCATCTAAACTCTCG Detection of ϕEf11 ORF 43
EF44F ACCAAGATTTGACGCAGAAGTTGCC Detection of ϕEf11 ORF 44
EF44R TGGCCATCGTCGTCTTTATCTGCT Detection of ϕEf11 ORF 44
EF60F AGACGTTTGGACCGAATAGCTGGT Detection of ϕEf11 ORF 60
EF60R TGCGGTAAGCTTCTGCGAATTCAA Detection of ϕEf11 ORF 60
Fl1A35F GGGAACTAGCAGTTGAAGAATCGC Detection of ϕFL1C gp40
Fl1A35R TTCCTTTGTACTATCTTGATCTCCA Detection of ϕFL1C gp40
Fl1A37F GAGCGTTTAGATAAGTCGGATTGG Detection of ϕFL1C gp44
Fl1A38R CCAAGTTTCTTTAGCCTGGTCACG Detection of ϕFL1C gp44