Table 2.

Individual Cq values of the candidate reference genes in healthy control and foot rot-affected sheep, with or without selenium (Se) treatment, and combined groups.

Gene symbol	Healthy control sheep (n = 4)		Foot rot-diseased sheep, untreated with Se (n = 6)		Foot rot-diseased sheep, treated with Se (n = 6)		Combined groups (n = 16)
	Mean Cq	SD	Mean Cq	SD	Mean Cq	SD	Mean Cq	SD
ACTB	18.68	0.88	18.14	0.52	19.09	0.36	18.63	0.69
RPL19	18.72	0.56	18.68	0.41	19.22	0.35	18.89	0.49
B2M	19.61	1.47	19.01	0.80	20.72	0.87	19.80	1.18
GAPDH	20.09	0.39	19.59	0.28	19.88	0.45	19.82	0.38
YWHAZ	20.82	0.35	20.48	0.33	20.60	0.37	20.61	0.35
SDHA	20.99	0.39	21.06	0.30	21.25	0.45	21.11	0.34
PGK1	21.12	0.51	21.24	0.53	21.58	0.41	21.34	0.51
G6PD	21.82	0.23	21.62	0.22	21.95	0.24	21.80	0.26
HPRT	25.54	2.33	23.76	1.86	24.59	2.07	24.52	2.22
TFRC	29.17	1.82	28.02	1.04	30.55	0.89	29.25	1.70
GYPC	29.56	0.59	30.24	0.19	30.15	0.71	30.04	0.55