Table 3.
Stability ranking of candidate reference genes in healthy control and foot rot-affected sheep, with or without selenium (Se) treatment, and in combined groups, by the geNorm algorithm (lower stability values indicate more stable gene expression).
| Healthy control sheep (n = 4) | Foot rot-diseased sheep, untreated with Se (n = 6) | Foot rot-diseased sheep, treated with Se (n = 6) | Combined groups (n = 16) | ||||
|---|---|---|---|---|---|---|---|
|
| |||||||
| Gene Symbol and Stability Value | |||||||
| SDHA|G6PD | 0.041 | GAPDH|YWHAZ | 0.235 | GAPDH|YWHAZ | 0.195 | GAPDH|YWHAZ | 0.278 |
| YWHAZ | 0.258 | G6PD | 0.253 | G6PD | 0.265 | G6PD | 0.307 |
| GAPDH | 0.333 | SDHA | 0.284 | SDHA | 0.288 | SDHA | 0.327 |
| RPL19 | 0.449 | RPL19 | 0.346 | RPL19 | 0.326 | RPL19 | 0.396 |
| PGK1 | 0.542 | PGK1 | 0.426 | ACTB | 0.420 | PGK1 | 0.478 |
| ACTB | 0.631 | ACTB | 0.495 | PGK1 | 0.466 | ACTB | 0.546 |
| B2M | 0.756 | B2M | 0.588 | B2M | 0.583 | B2M | 0.683 |