Table 4.
Stability ranking of candidate reference genes in healthy control and foot rot-affected sheep, with or without selenium (Se) treatment and in combined groups, by the NormFinder algorithm (lower stability values indicate more stable gene expression).
| Healthy control sheep (n = 4) | Foot rot-diseased sheep, untreated with Se (n = 6) | Foot rot-diseased sheep, treated with Se (n = 6) | Combined groups (n = 16) | ||||
|---|---|---|---|---|---|---|---|
|
| |||||||
| Gene Symbol and Stability Value | |||||||
| G6PD | 0.253 | G6PD | 0.127 | RPL19 | 0.183 | G6PD | 0.198 |
| SDHA | 0.265 | GAPDH | 0.209 | YWHAZ | 0.265 | RPL19 | 0.234 |
| RPL19 | 0.297 | YWHAZ | 0.249 | G6PD | 0.298 | GAPDH | 0.377 |
| GAPDH | 0.434 | RPL19 | 0.281 | ACTB | 0.333 | YWHAZ | 0.406 |
| YWHAZ | 0.528 | SDHA | 0.379 | GAPDH | 0.360 | SDHA | 0.433 |
| ACTB | 0.607 | ACTB | 0.474 | PGK1 | 0.383 | ACTB | 0.465 |
| PGK1 | 0.779 | PGK1 | 0.698 | SDHA | 0.536 | PGK1 | 0.594 |
| B2M | 1.078 | B2M | 0.828 | B2M | 0.897 | B2M | 1.043 |