Table 5.
Stability ranking of candidate reference genes in healthy control and foot rot-affected sheep, with or without selenium (Se) treatment, and in combined groups, by the BestKeeper algorithm (lower stability values indicate more stable gene expression).
| Healthy control sheep (n = 4) | Foot rot-diseased sheep, untreated with Se (n = 6) | Foot rot-diseased sheep, treated with Se (n = 6) | Combined groups (n = 16) | ||||
|---|---|---|---|---|---|---|---|
|
| |||||||
| Gene Symbol and Stability Value | |||||||
| GAPDH | 0.131 | G6PD | 0.153 | G6PD | 0.202 | G6PD | 0.226 |
| SDHA | 0.158 | GAPDH | 0.211 | RPL19 | 0.249 | YWHAZ | 0.282 |
| G6PD | 0.168 | YWHAZ | 0.220 | ACTB | 0.251 | SDHA | 0.291 |
| YWHAZ | 0.218 | SDHA | 0.220 | YWHAZ | 0.263 | GAPDH | 0.308 |
| PGK1 | 0.388 | RPL19 | 0.336 | PGK1 | 0.321 | RPL19 | 0.380 |
| RPL19 | 0.465 | PGK1 | 0.355 | SDHA | 0.346 | PGK1 | 0.392 |
| ACTB | 0.642 | ACTB | 0.377 | GAPDH | 0.354 | ACTB | 0.577 |
| B2M | 0.951 | B2M | 0.546 | B2M | 0.557 | B2M | 0.906 |