Table 7.
Stability ranking of candidate reference genes in healthy control and foot rot-affected sheep, with or without selenium (Se) treatment, and in combined groups, by the geomean of ranking values for geNorm, NormFinder, BestKeeper and the comparative delta Cq method (lower geomean values indicate more stable gene expression).
| Healthy control sheep (n = 4) | Foot rot-diseased sheep, untreated with Se (n = 6) | Foot rot-diseased sheep, treated with Se (n = 6) | Combined groups (n = 16) | ||||
|---|---|---|---|---|---|---|---|
|
| |||||||
| Gene Symbol and Geomean Ranking Value | |||||||
| G6PD | 1.46 | G6PD | 1.32 | YWHAZ | 1.86 | G6PD | 1.32 |
| SDHA | 1.86 | GAPDH | 1.86 | RPL19 | 2.11 | YWHAZ | 2.63 |
| GAPDH | 2.83 | YWHAZ | 2.52 | G6PD | 2.28 | GAPDH | 2.71 |
| RPL19 | 4.05 | RPL19 | 4.47 | GAPDH | 3.81 | RPL19 | 3.16 |
| YWHAZ | 4.16 | SDHA | 4.47 | ACTB | 4.53 | SDHA | 4.16 |
| PGK1 | 6.19 | PGK1 | 6.48 | PGK1 | 5.73 | PGK1 | 6.48 |
| ACTB | 6.48 | ACTB | 6.48 | SDHA | 5.86 | ACTB | 6.48 |
| B2M | 8.00 | B2M | 8.00 | B2M | 8.00 | B2M | 8.00 |